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Objective@#To study the genome molecular characteristics of Getah virus (SC1210) which isolated in Sichuan province in 2012.@*Methods@#Reverse transcriptase polymerase chain reaction (RT-PCR) was used to identify the isolate and the genome was sequenced by the second Ion Torrent PGM. Computer softwares, including Mega Align and Mega 6, were used to analyze the nucleotide and deduced amino acid sequence, and draw phylogenetic trees.@*Results@#SC1210 was identified as Getah virus. The full genome sequence was 11 690nt, the nucleotide and amino acid homology of the full sequence with other strains were 99.2%-99.7% and 96.5%-99.4%.The capsid protein of SC1210 consisting of 804 nucleotides, encoding 268 amino acids and the full-length of E2 protein, had 1 266 nucleotides, encoding 422 amino acids. The nucleotide homology of the capsid protein and the E2 protein with other strains were 94.9%-99.2% and 94.6%-99.6%, and the amino acid were 97%-99.6% and 97.1%-99.5%. The 3′ UTR of the virus included 402 nucleotides and there were three repeat sequence elements and 19 nucleotides conservation sequence.@*Conclusions@#The first GETV isolate SC1210 in Sichuan province has a closer relationship with Yunnan strain YN040 and a far genetic relationship with MM2021.
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Objective To explore the locating, parameter measurement and 3D display of nucleus accumbens in human brain in terms of digital anatomy .Methods The raw data of the head specimen of a 45-year-old male adult with 0.5mm as the section spacing was collected by using digital milling machine .Three hundreds images of continual cross sections containing brain were chosen and the segmentation of the caudate nucleus , putamen and nucleus accumbens was accomplished with Photoshop CS .The nucleus accumbens on the images of continual coronal section reconstruction were distinguished according to Harvard Medical School ’ s segment method to calculate the volume of nucleus accumbens and collect the correlative location information .The caudate nucleus , putamen and nucleus accumbens were 3D visualize with the software of Amira 3.1.1.Results The nucleus accumbens , the adjoining structure and the lesion target of nucleus accumbens were all clearly visible .The left nucleus accumbens volume was 972.5mm3 , and the right was 830.6mm3 .The 3D coordinate value was the left ( -11.0, 24.4, 1.3) and the right (9.3, 23.9, 1.7).Conclusion The digital anatomy of nucleus accumbens can distinctly display the nucleus accumbens , form and confirm it ’ s volume, location and adjoining area , which is useful to clinician .
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Objective To analyze the clinical effects aftet one year of LASIK for myopia. Methods According to the preoperative diopters of the myopia, the patients were divided into three groups, group Ⅰ: 57 eyes( < - 6.00D), group Ⅱ: 109 eyes( - 6.25～ - 10.00D) ,group Ⅲ :31 eyes( > - 10.25D～ - 15.00D). The patients were followed up 1 year. Re- sults In groups,the percentage of the uncorrected visual acuity equal or more than 1.0 was 94.3%,80.3%,42.0% re- spectivdy. The percentage of there sidual refractive error within ±0.50D was 99.3% ,85.7% ,59.7%. The spherical e- quivalent was stable in 3 months after LASIK. The complications are few. Conclusion LASIK is a safe,effective and good predictable method for treatment of myopia.
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Objective To evaluate the curative effect of LASIK for correcting compound myopic astigmatism.Methods One hundred and sixteen eyes in 68 patients with compound myopic astigmatism had been corrected with LASIK.All patients were followed up for more than 12 months.Meanwhile,all eyes were divided into two groups according to the preoperative astigmatism:group I (-2.25～ -3.00D),group Ⅱ (-3.2.5～ -6.00D).Results In group I :the average refractive cylinder was (- 2.51±0.68) D preoperafion, and had the average of (- 0.51±0.25) D residual astegmatism postoperation.In group Ⅱ :the average refractive cylinder was (- 3.85±0.46) D preoperation, and had the average of (- 0.52±0.22)D residual astigmatism postoperation.There was no statistical difference in astigmatism between the two groups after 12 months.In group Ⅰ and Ⅱ the visual acuity after operation was better than the corrected visual acuity before operation.Conclusion For compound myopic astigmatism LASIK was safe and effective.
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<p><b>OBJECTIVE</b>To study micro morphology and element-mixing distribution of different alloys welded in laser and analyze the feasibility of laser welding different alloys.</p><p><b>METHODS</b>Alloys and titanium were matched into 4 groups: Au-Pt with Ni-Cr; Au-Pt with pure Ti; pure Ti with Ni-Cr; Ni-Cr with Co-Cr. They were welded in laser. Changes in metallography after hybridization of crystalline grain, ranges of heat-affected zone and pores were observed through SEM with ultra-thin windowed X-ray energy atlas. Meanwhile 10 testing points were chosen with area of 300 micro m x 900 micro m along the welding surface from the side A alloy to the side B alloy, than the element mixing distribution and tendency were analyzed with X-ray energy atlas.</p><p><b>RESULTS</b>1. Hybridization of different alloys: (l) in the group of Au-Pt with Ti, there was titanium element mixing into Au-Pt tissue gradually and evenly on the Au-Pt side of the interface without clear boundary and increasing in size of crystalline grain. However, there was titanium crystalline grain increasing in size, irregular morphology and small sacks on the titanium side with clear boundary. (2) in the group of Ni-Cr with Ti, there was mixing regularly, slow transition and interlocks between crystalline grains on the Ni-Cr side of the in terface. Poor transition, clear boundary and small cracks were observed on titanium side. (3) in the group of Co-Cr with Ni-Cr, there was good transition, obscure boundary on both sides resulting from network, cylinder and branch structure growing. 2. Element-mixing distribution of different alloys. In fusion zone, the metal elements in matched groups mixed well and hybridized into new alloys except titanium blocks. The location of wave peak depended on the composition of alloys. Most of elements were from the alloy far from the fusion zone.</p><p><b>CONCLUSION</b>The hybridization between pure titanium and any other alloys is not good The effect of laser welding different alloys is ideal except with pure titanium.</p>